IQUIMEFA 05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
artículos
Título:
New insights into antibacterial and antioxidant activities of rosemary essential oils and their main components
Autor/es:
A.M. OJEDA-SANA; C.M. VAN BAREN; M.A. ELECHOSA; M.A. JUÁREZ; S. MORENO
Revista:
FOOD CONTROL
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 31 p. 189 - 195
ISSN:
0956-7135
Resumen:
Rosemary essential oils constituents were investigated by GC-FID-MS. Microbiological analysis using the disk diffusion and microplate bioassay as well as fluorimetric approaches by SYTOX Green stain to study bacterial membrane permeabilization were performed. The antibiotic activity of rosemary oils containing α-pinene, myrcene, 1,8-cineole, borneol and camphor as major constituents, was lower than the activity of pure constituents displaying minimal inhibition concentration, MIC, 10 to 25 μl ml-1 and 0.8 to 8 μl ml-1, respectively. α-Pinene is the main bioactive showing the broadest activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli. 1,8-cineole was bactericidal on E. coli trough the cell membrane permeabilization, effect that it was demonstrated for the first time using a fluorimetric analysis.
