Preparation of submicrometer monodisperse magnetic silica particles using a novel water in oil microemulsion: properties and application for enzyme immobilization

MV TUTTOLOMONDO; ME VILLANUEVA; ALVAREZ G.; DESIMONE, M.F.; DIAZ, L.E

BIOTECHNOLOGY LETTERS

SPRINGER

Lugar: Berlin; Año: 2013 p. 1571 - 1577

0141-5492

Enzyme immobilization has attracted great interest especially because it facilitates theseparation from products and improves of enzyme activity and stability. Herein, wedescribe the synthesis of monodisperse magnetic silica nanoparticles (MSNs), usingW/O reverse emulsion system that does not require the use of cosurfactants. Wepropose the use of a sodium silicate solution, Tween 20 as a neutral surfactant and 1-butanol as the organic phase. There are several advantages of the proposed methodincluding the higher levels of magnetization of the particles obtained, uniformity of sizeand that they are easy functionalized to covalently bound urease. Moreover, theintraday, interday and long term stability results confirm that the procedure wassuccessful and the enzyme linked MSNs are stable over repeated uses and storage