IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
artículos
Título:
Involvement of membrane tubulin in erythrocyte
Autor/es:
MARIANA AMAIDEN; NOELIA MONESTEROLO; VERÓNICA SANTANDER; ALEXIS CAMPETELLI; CARLOS ARCE; JUAN PIE; SANDRA I HOPE; MARCELO S. VATTA; CESAR CASALE
Revista:
JOURNAL OF HYPERTENSION
Editorial:
LIPPINCOTT WILLIAMS & WILKINS
Referencias:
Lugar: London; Año: 2012 vol. 30 p. 1414 - 1422
ISSN:
0263-6352
Resumen:
Objective: To test the hypothesis that erythrocyte
deformability is influenced by changes in the content of
membrane tubulin (Mem-tub).To test the hypothesis that erythrocyte
deformability is influenced by changes in the content of
membrane tubulin (Mem-tub).
Methods and results: Human erythrocytes contain
tubulin distributed in three pools (membrane,
sedimentable, soluble). Erythrocytes from hypertensive
humans have a higher proportion of Mem-tub. Increased
Mem-tub content in hypertensive patients was correlated
with decreased erythrocyte deformability. Treatment of
erythrocytes from normotensive individuals with taxol
increased Mem-tub content and reduced deformability,
whereas treatment of hypertensive patients erythrocytes
with nocodazole had the opposite effect. In-vivo
experiments with rats were performed to examine the
possible relationship between Mem-tub content,
erythrocyte deformability, and blood pressure.
Spontaneously hypertensive rats (SHRs) showed lower
erythrocyte deformability than normotensive Wistar rats.
During the development of hypertension in SHR, tubulin in
erythrocytes is translocated to the membrane, and this
process is correlated with decreased deformability. In-vivo
treatment (intraperitoneal injection) of SHR with
nocodazole decreased Mem-tub content, increased
erythrocyte deformability, and decreased blood pressure,
whereas treatment of Wistar rats with taxol had the
opposite effects.Human erythrocytes contain
tubulin distributed in three pools (membrane,
sedimentable, soluble). Erythrocytes from hypertensive
humans have a higher proportion of Mem-tub. Increased
Mem-tub content in hypertensive patients was correlated
with decreased erythrocyte deformability. Treatment of
erythrocytes from normotensive individuals with taxol
increased Mem-tub content and reduced deformability,
whereas treatment of hypertensive patients erythrocytes
with nocodazole had the opposite effect. In-vivo
experiments with rats were performed to examine the
possible relationship between Mem-tub content,
erythrocyte deformability, and blood pressure.
Spontaneously hypertensive rats (SHRs) showed lower
erythrocyte deformability than normotensive Wistar rats.
During the development of hypertension in SHR, tubulin in
erythrocytes is translocated to the membrane, and this
process is correlated with decreased deformability. In-vivo
treatment (intraperitoneal injection) of SHR with
nocodazole decreased Mem-tub content, increased
erythrocyte deformability, and decreased blood pressure,
whereas treatment of Wistar rats with taxol had the
opposite effects.
Conclusion: These findings indicate that increased Memtub
content contributes to reduced erythrocyte
deformability in hypertensive animals.These findings indicate that increased Memtub
content contributes to reduced erythrocyte
deformability in hypertensive animals.