Effects of postconditioning on ischaemic-reperfused hearts from fed and fasted rats

M G MARINA PRENDES, R HERMANN, M E TORRESIN, P SOUTO, S TALLIS, E A SAVINO AND A VARELA.

JOURNAL OF PHYSIOLOGICAL SCIENCES

SPRINGER TOKYO

Año: 2011 vol. 62 p. 333 - 341

1880-6546

The effects of ischemic-postconditioning (IPOC) on functional recovery and cell viability of ischemic-reperfused hearts from fed and fasted rats were studied in relation to triacylglycerol and glycogen mobilization, ATP content, glucose-6-phosphate-dehydrogenase activity and reduced/oxidized glutathion (GSH/GSSG). Oxidative damage was estimated by measuring thiobarbituric-acid-reactive-substances (TBARS). IPOC improved contractile recovery and cell viability in the fed but attenuated them in the fasted hearts. In both groups ischemia lowered glycogen. IPOC further reduced it. Triacylglycerol remained unchanged during ischemia-reperfusion in both groups, but triacylglycerol mobilization was activated by IPOC in the fasted. ATP was increased by IPOC in the fed hearts, but lowered in the fasted, which appeared to be associated with the rates of ATP synthesis in isolated mitochondria. In the fed hearts IPOC raised glucose-6-phosphate-dehydrogenase-activity and GSH/GSSG, and lowered TBARS. These results suggest that IPOC effects would be associated with changes in ATP supply, mobilization of energy sources and glutathione antioxidant ratio.