Optimization and Validation of the Quantitative Assay of Flavonoids in Achyrocline satureioides and A. flaccida

DAIANA S. RETTA; PAULA G. LÓPEZ; MARTHA A. GATTUSO; SUSANA J. GATTUSO; ROSANA FILIP; GRACIELA E. FERRARO; ARNALDO L. BANDONI

ACTA FARM. BONAERENSE

COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES

Lugar: Buenos Aires; Año: 2011 vol. 30 p. 1360 - 1365

0326-2383

SUMMARY. Several populations of Achyrocline satureioides and Achyrocline flaccida from Argentina, twoaromatic herbal species widely used in traditional medicine in South America and both known as marcela,were analyzed. The aims of this work were to evaluate the amounts of flavonoids that characterize thesespecies in this country and provide a quantitative assay to be included in the monograph of marcela for futureArgentine Pharmacopoeia editions. The extraction method and analysis by HPLC of the mainflavonoids, quercetin and 3-O-methylquercetin, were optimized. The validation parameters of the methodwere determined. The analysis of the different parts of these plants was carried out thereafter. Inflorescenceswere the parts displaying the highest content of such flavonoids. It was found that A. flaccida had aslightly higher content of flavonoids than A. satureioides (1.2 ± 0.4 % of quercetin, 0.8 ± 0.3 % of 3-Omethylquercetin;0.8 ± 0.2 % of quercetin and 0.7 ± 0.5 % of 3-O-methylquercetin, respectively).