Egg white lysozyme puriWcation with a chitin–silica-based aYnity

FEDERICO J. WOLMAN; GUILLERMO J. COPELLO; ANDREA M. MEBERT; ALEXANDRA M. TARGOVNIK; MARÍA V. MIRANDA; AGUSTÍN A. NAVARRO DEL CAÑIZO ·; LUIS E. DÍAZ; OSVALDO CASCONE

EUROPEAN FOOD RESEARCH AND TECHNOLOGY

SPRINGER

Lugar: Londres; Año: 2010 vol. 231 p. 181 - 188

1438-2377

Abstract A composite biosorbent retaining noncovalently bound chitin in between layers of a silicon oxide matrix was assessed for lysozyme puriWcation from undiluted egg white. The matrix can be shaped with big size and high density, thus allowing its eYcient separation from the egg white after the adsorption step. The lysozyme-depleted egg white can follow its usual commercialisation route. A surface area of 142 m2/g and a total pore volume of 0.295 cm3/g were calculated from the nitrogen sorption isotherms.Its water content was 78.3%. The matrix structure is the result of the polysaccharide addition to the polymerisation mixture, which is known to inXuence the condensation process, leading to a material with characteristic properties. A maximum capacity of 117.1 § 9 mg lysozyme/ g and a dissociation constant of 0.73 § 0.15 mg/mL were calculated from the Langmuir isotherm. A lysozyme puriWcation batch process from undiluted egg white was developed, where 87% of the lysozyme was removed from the egg white and the matrix was easily recovered by a simple Wltration through a strainer. The overall yield of the process was 64% with a puriWcation factor of 20. matrix was assessed for lysozyme puriWcation from undiluted egg white. The matrix can be shaped with big size and high density, thus allowing its eYcient separation from the egg white after the adsorption step. The lysozyme-depleted egg white can follow its usual commercialisation route. A surface area of 142 m2/g and a total pore volume of 0.295 cm3/g were calculated from the nitrogen sorption isotherms.Its water content was 78.3%. The matrix structure is the result of the polysaccharide addition to the polymerisation mixture, which is known to inXuence the condensation process, leading to a material with characteristic properties. A maximum capacity of 117.1 § 9 mg lysozyme/ g and a dissociation constant of 0.73 § 0.15 mg/mL were calculated from the Langmuir isotherm. A lysozyme puriWcation batch process from undiluted egg white was developed, where 87% of the lysozyme was removed from the egg white and the matrix was easily recovered by a simple Wltration through a strainer. The overall yield of the process was 64% with a puriWcation factor of 20. matrix was assessed for lysozyme puriWcation from undiluted egg white. The matrix can be shaped with big size and high density, thus allowing its eYcient separation from the egg white after the adsorption step. The lysozyme-depleted egg white can follow its usual commercialisation route. A surface area of 142 m2/g and a total pore volume of 0.295 cm3/g were calculated from the nitrogen sorption isotherms.Its water content was 78.3%. The matrix structure is the result of the polysaccharide addition to the polymerisation mixture, which is known to inXuence the condensation process, leading to a material with characteristic properties. A maximum capacity of 117.1 § 9 mg lysozyme/ g and a dissociation constant of 0.73 § 0.15 mg/mL were calculated from the Langmuir isotherm. A lysozyme puriWcation batch process from undiluted egg white was developed, where 87% of the lysozyme was removed from the egg white and the matrix was easily recovered by a simple Wltration through a strainer. The overall yield of the process was 64% with a puriWcation factor of 20. A composite biosorbent retaining noncovalently bound chitin in between layers of a silicon oxide matrix was assessed for lysozyme puriWcation from undiluted egg white. The matrix can be shaped with big size and high density, thus allowing its eYcient separation from the egg white after the adsorption step. The lysozyme-depleted egg white can follow its usual commercialisation route. A surface area of 142 m2/g and a total pore volume of 0.295 cm3/g were calculated from the nitrogen sorption isotherms.Its water content was 78.3%. The matrix structure is the result of the polysaccharide addition to the polymerisation mixture, which is known to inXuence the condensation process, leading to a material with characteristic properties. A maximum capacity of 117.1 § 9 mg lysozyme/ g and a dissociation constant of 0.73 § 0.15 mg/mL were calculated from the Langmuir isotherm. A lysozyme puriWcation batch process from undiluted egg white was developed, where 87% of the lysozyme was removed from the egg white and the matrix was easily recovered by a simple Wltration through a strainer. The overall yield of the process was 64% with a puriWcation factor of 20.Wcation from undiluted egg white. The matrix can be shaped with big size and high density, thus allowing its eYcient separation from the egg white after the adsorption step. The lysozyme-depleted egg white can follow its usual commercialisation route. A surface area of 142 m2/g and a total pore volume of 0.295 cm3/g were calculated from the nitrogen sorption isotherms.Its water content was 78.3%. The matrix structure is the result of the polysaccharide addition to the polymerisation mixture, which is known to inXuence the condensation process, leading to a material with characteristic properties. A maximum capacity of 117.1 § 9 mg lysozyme/ g and a dissociation constant of 0.73 § 0.15 mg/mL were calculated from the Langmuir isotherm. A lysozyme puriWcation batch process from undiluted egg white was developed, where 87% of the lysozyme was removed from the egg white and the matrix was easily recovered by a simple Wltration through a strainer. The overall yield of the process was 64% with a puriWcation factor of 20.