CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Tyrosine hydroxylase in Apidae and Triatominae
Autor/es:
GALVANI GL; CANAVOSO LE; SETTEMBRINI, B; NOWICKI S; LEYRA J
Lugar:
Orlando
Reunión:
Congreso; XXIV International Congress of Entomology; 2016
Institución organizadora:
Entomological Society of America
Resumen:
Tyrosine hydroxylase (TH) catalyzes the rate limiting step of cathecolamine biosynthesis. In insects, cathecholamines are involved in locomotion, learning and cuticle sclerotization among other functions. Two isoforms of TH have been reported; the shorter one which lacks a highly acidic region is present in neural cells.The distribution of TH was studied in the nervous system of triatomine bugs (Triatoma infestans)and bees (Melitoma segmentaria, Thygater analis and Leiopodus lacertinus) using our current immunocytochemistry protocol. The TH antiserum developed by O? Gorman et al. (2007) in Manduca sexta was employed in the immunolocalization of cell bodies as well as in western blots.This TH antiserum allowed the detection of immunoreactive (IR) somata and eurites in the brain of all the species above mentioned. Most of the immunopositive cell bodies displayed a uniform size of about 10 µm. In the protocerebrum, clusters with variable numbers of somata were observedabove the mushroom body calices, in the pars intercerebralis as well as around the central complex.The antennal lobe glomeruli and the dorsal lobe also housed immunoreactive elements in all these species. It was found that within the bee species, only L. lacertinus contained TH-IR cell bodies in the proximal optic lobe, a finding also shared by triatomine bugs.The anti-TH antibody recognized an immunoreactive band of ~60kDa, compatible with a predicted mass for the monomer form of 66.3 kDa. The presence of TH in the same brain areas of insect species from separate orders suggests highly conserved functions for catecholamines.