Arabidopsis thaliana seeds with high allantoin content increases germination rate under saline condition

MARTINI, C; TESSI, TM; RODRIGUEZ, C; LESCANO, CI; DESIMONE M

Congreso; XVI CONGRESO LATINOAMERICANO DE FISIOLOGIA VEGETAL; 2018

Allantoin is a nitrogenous compound derived from the purines oxidation pathway. Numerous studies have shown a close relationship between the activation of this pathway and plant stress response. In the present work, the effect of allantoin on Arabidopsis thaliana seed germination under salinity condition was analy- zed. To address this objective, the germination rate of WT and transgenic lines aln1 and pUBI10: ALN / WT (knockout and overexpressing line of the enzyme alan- toinase, respectively) was measured. Besides, germi- nation rate and allantoin content of WT seeds formed under salinity conditions were studied. Germination tests were carried out in MS (Murashige and Skoog Basal Salt Mixture) medium 0.5X and agar plus the co- rresponding treatment (0 and 100 mM NaCl with or wit- hout 10 mM allantoin).Addition of 10 mM allantoin improved the germination rate of WT seeds under 100 mM NaCl. On the other hand, aln1 mutant which accumulated constitutively allantoin, showed higher germination rates in 100 mM NaCl when compared to WT and pUBI10: ALN / WT. In- terestingly, seeds formed in WT plants irrigated with NaCl during flowering time, showed higher allantoin content and better germination rates in saline medium than control seeds.