In vitro viral inactivation photosensitized by a natural anthraquinones

MUGAS L; CABRERA JL; KONIGHEIM BS; CONTIGIANI MS; AGUILAR JJ; NÚÑEZ MONTOYA SC

Villa Carlos Paz

Encuentro; XIII ENCUENTRO LATINOAMERICANO DE FOTOQUÍMICA Y FOTOBIOLOGÍA (ELAFOT); 2017

Grupo Argentino de Fotobiología

From the phototoxic plant, Heterophyllaea pustulata Hook. f. (Rubiaceae), a bush that grows in the Andean northwest of Argentina, we have obtained 9,10-anthraquinone derivatives (AQs) with photosensitizing properties [1-3]. Previously, we have demonstrated that these AQs show in vitro virucidal effect against Herpes Simplex virus Type 1 (HSV-1), which means an inactivation of viral particles before their entry into the host cells, and this inhibition was increased by light action (photostimulation) [4]. The aim of this work was study the in vitro inactivation of HSV-1 once the virus entered into the host-cells, by the photosensitized action of two natural AQs: soranjidiol (Sor) and 5,5´-bisoranjidiol (Bisor), a soranjidiol dimer.Isolated AQs were identified by their RMN spectra. To test the viral inactivation effect of each AQ on infected cells, it is previously necessary to find the concentrations of each AQ that are not toxic for the host cells (cytotoxic assay). In the two assays, morphological cellular alterations were evaluated by optical microscopic (cytopathic effect, CPE) and cellular viability (CV) was determined by uptake NR assay, under two simultaneous conditions: darkness and irradiation (actinic lamp 380-480 nm, Philips TL/03). Cytotoxic test was performed in a monolayer of Vero cells (host cells) treated with different concentrations of each AQ, dissolved in MEM with DMSO