The antithrombotic in vivo effects of quercetin-tetra-sulphate (QTS) isolated from Flaveria bidentis in an experimental thrombosis model in mice

CUADRA GR ; A.M. AGNESE; CABRERA JL ; GUGLIELMONE HA; NUÑEZ-MONTOYA SC

Berlín

Congreso; XXVI Congress of the International Society on Thrombosis and Haemostasis; 2017

Objective Quercetin-tetra-sulphate (QTS) has been shown to inhibit ?in vitro? platelet aggregation and also possesses important anticoagulant, and profibrinolytic activities. In this report, we examined ?in vivo? the antithrombotic activity of QTS in an experimental thrombosis model in mice using an acute pulmonary thromboembolism test.Methods Acute pulmonary thromboembolism was induced, after QTS treatment at different concentrations, by an intravenous injection of a collagen and epinephrine mixture. The paralyzed and dead mice in each group were numbered and the percentage of protection against acute pulmonary thromboembolism was calculated. Also, the tail bleeding assay and the bleeding loss were determined in the presence and absence of QTS at 100, 50, 25, and 10 mg/kg and compared with acetylsalicylic acid (ASA) treated mice. The histologic study was conducted in lung tissue to estimate the percentage of opened and occluded vessels by microthrombi.Results QTS (100; 50; and 25 mg/kg), administered i.p in mice, showed antithrombotic activity preventing the paralysis or death (90, 90 and 70%, respectively) induced by the collagen?epinephrine intravenous injection. The positive control (ASA 50 mg/kg) was able to protect to 70%. The tail bleeding test and the blood loss were estimated and a profile dose-dependent was observed. This observation was confirmed by the lung histologic examination, in which the density of not occluded blood vessels was significantly decreased (92; 77 % and 31%; p < 0.01) compared to vehicle treated mice.Conclusion We suggest that QTS should be an interesting natural compound for the treatment in thrombotic disease.