IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Quantitative analysis of boldine alkaloid in natural extracts by cyclicvoltammetry at a liquidliquid interface and validation of the method by comparison with high performance liquid chromatography
Autor/es:
C.I. CÁMARA; C.A. BORNANCINI; J.L. CABRERA; M.G. ORTEGAB; L.M. YUDI
Revista:
TALANTA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2010 vol. 84 p. 623 - 630
ISSN:
0039-9140
Resumen:
The quantitative determination of boldine alkaloid in boldo leaf extracts by employing cyclic voltammetry,
at a liquid/liquid interface as well as the validation of this methodology against the reference
method, high performance liquid chromatography (HPLC), are reported in the present paper. The voltammetric
analysis was performed successfully and economically using two kinds of liquid/liquid interfaces:
water/1,2-dicholoroethane and water/PVC (polyvinyl chloride)gelled 1,2-dichloroethane. Linear calibration
curves in the concentration range of 1.04×10−5 mol L−1 to 5.19×10−4 mol L−1 were obtained
with a detection limit equal to (6.1±0.7)×10−5 mol L−1 and the quantitative determination of this alkaloid,
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
with a detection limit equal to (6.1±0.7)×10−5 mol L−1 and the quantitative determination of this alkaloid,
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
with a detection limit equal to (6.1±0.7)×10−5 mol L−1 and the quantitative determination of this alkaloid,
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
×10−5 mol L−1 to 5.19×10−4 mol L−1 were obtained
with a detection limit equal to (6.1±0.7)×10−5 mol L−1 and the quantitative determination of this alkaloid,
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
±0.7)×10−5 mol L−1 and the quantitative determination of this alkaloid,
in complex matrixes such as boldo leaf extracts, by the electrochemical technique proposed was
found to be equal to the values obtained using the standard HPLC method. The validation analysis of
this methodology against HPLC demonstrated that accuracy, linearity, limit of detection (LOD), limit of
quantification (LOQ), specificity and precision are acceptable. The electroanalytical technique proposed
is economical and selective, involves simple equipment and can be applied for the quantitative determination
of boldine alkaloid in complex matrixes such as leaf extracts without special drug separation.
Moreover, cyclic voltammetry (CV) experiments applied at the liquid/liquid interface under different
experimental conditions allowed us to study the transfer mechanism of boldine, and determine a value
of pKa
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.
Ka
w = 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.= 6.90 for protonated boldine, from the variation of voltammetric peak current with pH.