IMBIV 05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Study of amino ligands fixation to macroporous supports and their influence on albumin adsorption
Autor/es:
CESAR GOMEZ; MIRIAM STRUMIA
Revista:
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
Editorial:
JOHN WILEY & SONS INC
Referencias:
Año: 2009 p. 6771 - 6782
ISSN:
0887-624X
Resumen:
Heterogeneous networks of ethylene glycol dimethacrylate and 2-hydroxyethyl
methacrylate [poly(EGDMA-co-HEMA)] were synthesized by suspension polymerization
using different EGDMA contents and agitation speeds. The networks were
actived with epichlorhydrine (Ech) or 1,4-butanediol diglycidyl ether (BDGE), and
then hexamethylenediamine (HMDA) or ethylenediamine (EDA) were conjugated to
the support by coupling reaction. Here, a higher alkyldiamine concentration and temperature,
and a longer reaction time led to higher yields. Amino ligands of the support
III were used to analyze their adsorption performance of bovine serum albumin (BSA)
from the adsorption kinetic. A more external location of HMDA amino ligands into network
led to get the maximum adsorption in a time shorter than that with EDA. Due
to its bigger size, the HMDA molecule was attached mostly to the network surface
between larger pores, which favored a faster protein adsorption. When derivatives containing
BDGE were compared, the EDA ligand displayed a BSA retention higher than
that with HMDA, because a shorter separation between the ammonium groups along
the spacer arm yielded a stronger electrostatic attraction on the protein. Clearly, the
balance obtained between the pores system and the reagents molecular structure used
in the formation of Ech-HMDA generated the most efficient BSA adsorption
using different EGDMA contents and agitation speeds. The networks were
actived with epichlorhydrine (Ech) or 1,4-butanediol diglycidyl ether (BDGE), and
then hexamethylenediamine (HMDA) or ethylenediamine (EDA) were conjugated to
the support by coupling reaction. Here, a higher alkyldiamine concentration and temperature,
and a longer reaction time led to higher yields. Amino ligands of the support
III were used to analyze their adsorption performance of bovine serum albumin (BSA)
from the adsorption kinetic. A more external location of HMDA amino ligands into network
led to get the maximum adsorption in a time shorter than that with EDA. Due
to its bigger size, the HMDA molecule was attached mostly to the network surface
between larger pores, which favored a faster protein adsorption. When derivatives containing
BDGE were compared, the EDA ligand displayed a BSA retention higher than
that with HMDA, because a shorter separation between the ammonium groups along
the spacer arm yielded a stronger electrostatic attraction on the protein. Clearly, the
balance obtained between the pores system and the reagents molecular structure used
in the formation of Ech-HMDA generated the most efficient BSA adsorption
co-HEMA)] were synthesized by suspension polymerization
using different EGDMA contents and agitation speeds. The networks were
actived with epichlorhydrine (Ech) or 1,4-butanediol diglycidyl ether (BDGE), and
then hexamethylenediamine (HMDA) or ethylenediamine (EDA) were conjugated to
the support by coupling reaction. Here, a higher alkyldiamine concentration and temperature,
and a longer reaction time led to higher yields. Amino ligands of the support
III were used to analyze their adsorption performance of bovine serum albumin (BSA)
from the adsorption kinetic. A more external location of HMDA amino ligands into network
led to get the maximum adsorption in a time shorter than that with EDA. Due
to its bigger size, the HMDA molecule was attached mostly to the network surface
between larger pores, which favored a faster protein adsorption. When derivatives containing
BDGE were compared, the EDA ligand displayed a BSA retention higher than
that with HMDA, because a shorter separation between the ammonium groups along
the spacer arm yielded a stronger electrostatic attraction on the protein. Clearly, the
balance obtained between the pores system and the reagents molecular structure used
in the formation of Ech-HMDA generated the most efficient BSA adsorption
