Rol of hyphal morphology and conidia adhesion on pellets formation in

PERA LICIA; COLIN VERÓNICA; ROMERO CINTIA AND BAGORÍ MARIO

Hotel Catalina Park, Tucumán-Argentina

Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009

The morphology on filamentous fungi in submerged culture affects the broth rheology and product yield. It is therefore crucial to understand how environmental conditions influence mycelial morphology. The aim of this work was establish the relationship between A. niger MYA 135 conidia adhesion and hyphal morphology on pellets formation. Microorganism was cultivated in basic medium at 30º C and initial pH 5. The modifications in the environmental conditions was tested by changing either the incubation temperature, the initial pH or by the addition of either CaCl2 or FeCl3 to medium. Conidia adhesion was quantify in 96-well polystirene microtiter plates by using sulforhodamine B staining. The adhesion unit was defined as the amount of adherent substance that produce an increase in absorbance at 570 nm of 0.01 per min per ml of culture. The hyphal growth unit length (lHGU) was calculated by dividing the total length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 quantify in 96-well polystirene microtiter plates by using sulforhodamine B staining. The adhesion unit was defined as the amount of adherent substance that produce an increase in absorbance at 570 nm of 0.01 per min per ml of culture. The hyphal growth unit length (lHGU) was calculated by dividing the total length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 quantify in 96-well polystirene microtiter plates by using sulforhodamine B staining. The adhesion unit was defined as the amount of adherent substance that produce an increase in absorbance at 570 nm of 0.01 per min per ml of culture. The hyphal growth unit length (lHGU) was calculated by dividing the total length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 hyphal morphology on pellets formation. Microorganism was cultivated in basic medium at 30º C and initial pH 5. The modifications in the environmental conditions was tested by changing either the incubation temperature, the initial pH or by the addition of either CaCl2 or FeCl3 to medium. Conidia adhesion was quantify in 96-well polystirene microtiter plates by using sulforhodamine B staining. The adhesion unit was defined as the amount of adherent substance that produce an increase in absorbance at 570 nm of 0.01 per min per ml of culture. The hyphal growth unit length (lHGU) was calculated by dividing the total length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 quantify in 96-well polystirene microtiter plates by using sulforhodamine B staining. The adhesion unit was defined as the amount of adherent substance that produce an increase in absorbance at 570 nm of 0.01 per min per ml of culture. The hyphal growth unit length (lHGU) was calculated by dividing the total length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 quantify in 96-well polystirene microtiter plates by using sulforhodamine B staining. The adhesion unit was defined as the amount of adherent substance that produce an increase in absorbance at 570 nm of 0.01 per min per ml of culture. The hyphal growth unit length (lHGU) was calculated by dividing the total length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 hyphal morphology on pellets formation. Microorganism was cultivated in basic medium at 30º C and initial pH 5. The modifications in the environmental conditions was tested by changing either the incubation temperature, the initial pH or by the addition of either CaCl2 or FeCl3 to medium. Conidia adhesion was quantify in 96-well polystirene microtiter plates by using sulforhodamine B staining. The adhesion unit was defined as the amount of adherent substance that produce an increase in absorbance at 570 nm of 0.01 per min per ml of culture. The hyphal growth unit length (lHGU) was calculated by dividing the total length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 quantify in 96-well polystirene microtiter plates by using sulforhodamine B staining. The adhesion unit was defined as the amount of adherent substance that produce an increase in absorbance at 570 nm of 0.01 per min per ml of culture. The hyphal growth unit length (lHGU) was calculated by dividing the total length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 length for each organism by the number of tips. Conidia adhesion units per ml higher than 0.50 could be necessary to afford pellets formation. In addition, it seems that once the pellet was formed the lHGU had influence on its final diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 diameter. Multiple linear regression with pellets diameters determined at 72 h of incubation versus conidia adhesion (6h) and lHGU explains 82.6 % of the variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICET and CIUNT 26/D409 variability of the date. This work was supported by grants 693/04 CONICE