PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Culture media optimization for Reactive Black 5 decolourization by Trichosporon porosum MM4029
Autor/es:
VIEJOBUENO, J; MARTORELL, MARÍA M.; FERNÁNDEZ, PABLO M; PAJOT, HIPÓLITO F.; FIGUEROA, LUCÍA I. C.
Lugar:
Wisconsin
Reunión:
Congreso; ICY 2012 Yeast for a sustainable future; 2012
Resumen:
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Textile
dyes are engineered to be resistant to environmental conditions. During the
last years the treatment of textile dye effluents has been the focus of
significant research because of the potentially low cost of the process. Mechanisms
of biological textile dyes decolourization greatly depend on the dye chemical
structure, the employed microorganisms and media culture composition. In this study,
decolourization of the diazo dye Reactive Black 5 was carried out by the native
yeast Trichosporon porosum 4029. The
effect of peptone, CaCl2, KH2PO4, MgSO4, glucose,
yeast extract and (NH4)2SO4 were initially studied
in a seven factor Plackett?Burman design. Only glucose, yeast extract and (NH4)2SO4
showed a significant effect on dye decolourization; whereas peptone, CaCl2,
KH2PO4 and MgSO4 could be safely excluded from
decolourization medium. Subsequently, a three factor full factorial design was employed
in order to study the effect and interactions between glucose, yeast extract and
(NH4)2SO4 concentration on dye decolourization.
In optimized medium, glucose and (NH4)2SO4
final concentrations were decreased to 10 g/l and 1.25 g/l, whereas yeast extract
final concentration was reduced to a 1 g/l. Those values represent a 60 %
reduction for the initial concentration of yeast extract and 50 % for the other
two components. Under those optimized conditions, Trichosporon porosum was able to decolourize almost completely 200
mg/l of Reactive Black 5 within 24 h. Results has allowed the formulation of a
new decolourization medium, simpler and greatly reduced in costs, for its
future use in bioremediation processes.