PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Screening for statin-producing ability in filamentous fungi isolated from subtropical rainforest (Yungas) of Tucumán, northwestern Argentina
Autor/es:
CABRAL MARÍA EUGENIA; DELGADO O,; SAMPIETRO DIEGO; CASTALÁN C. A.; CASTELLANOS DE FIGUEROA, LUCÍA INÉS; FARIÑA J.I.,
Revista:
MICROBIOLOGICAL RESEARCH
Editorial:
Elsevier B.V.
Referencias:
Lugar: Amsterdam, The Netherlands; Año: 2008 p. 1 - 1
ISSN:
0944-5013
Resumen:
Abstract: Research was aimed at
isolating and identifying fungal species from Las Yungas Pedemontana Forest
with ability for statin production. Fungal isolates were screened by yeast growth
inhibition bioassays, TLC and RP-HPLC analyses. Waring-blender-obtained fungal
extracts led to higher statin yields than after disrupting with glass-beads or
by freeze-thawing. Only 23 out of 230 isolates showed to be TLC(+) potential
statin-producers. Eighteen extracts inhibited S. cerevisiae ATCC 32051 during
yeast bioassays, while nine had the same effect on Candida utilis Pr1-2. Peaks
revealed by RP-HPLC corresponded to or were closely related to known statins. Promising
fungal isolates were identified as Hypocrea and Penicillium genera members. Statin
concentrations were in the order of those reported for wild-type strains under
non-optimal production conditions. The potential of Las Yungas filamentous
fungi for statin production under laboratory conditions could be evidenced. Selected
isolates may be considered as promising targets for optimizing statin
production. This is the first report where investigation focuses on indigenous
filamentous fungi from Las Yungas to give an insight in their hidden
biotechnological potential for statin production. Optimized protocols for
statin-production assessment are also proposed for future screening programs.