Enzymatic synthesis of banana flavour (isoamyl acetate) by B. licheniformis S-86 esterase

TORRES S; BAIGORI MARIO D.; SWATHY S.L.; ASHOK PANDEY; CASTRO G.R

FOOD RESEARCH INTERNATIONAL

ELSEVIER

Año: 2009

0963-9969

New carboxylesterase from organic-solvent-tolerant Bacillus licheniformis S-86 strain was characterized. The enzyme named as type II esterase showed an optimal activity in the temperature range 60-65°C and pH 8.0. The enzyme was moderatly thermostable (half-life of 1h at 50°C), but remarkable stable at extremely alkaline pH, retaining 100% of its activity at pH 10.0-11.0. Furthermore, the esterase showed high stability in detergents (86% residual activity in 10% SDS), and also 0.1 % ionic and non-ionic detergents are inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. characterized. The enzyme named as type II esterase showed an optimal activity in the temperature range 60-65°C and pH 8.0. The enzyme was moderatly thermostable (half-life of 1h at 50°C), but remarkable stable at extremely alkaline pH, retaining 100% of its activity at pH 10.0-11.0. Furthermore, the esterase showed high stability in detergents (86% residual activity in 10% SDS), and also 0.1 % ionic and non-ionic detergents are inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. characterized. The enzyme named as type II esterase showed an optimal activity in the temperature range 60-65°C and pH 8.0. The enzyme was moderatly thermostable (half-life of 1h at 50°C), but remarkable stable at extremely alkaline pH, retaining 100% of its activity at pH 10.0-11.0. Furthermore, the esterase showed high stability in detergents (86% residual activity in 10% SDS), and also 0.1 % ionic and non-ionic detergents are inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. Bacillus licheniformis S-86 strain was characterized. The enzyme named as type II esterase showed an optimal activity in the temperature range 60-65°C and pH 8.0. The enzyme was moderatly thermostable (half-life of 1h at 50°C), but remarkable stable at extremely alkaline pH, retaining 100% of its activity at pH 10.0-11.0. Furthermore, the esterase showed high stability in detergents (86% residual activity in 10% SDS), and also 0.1 % ionic and non-ionic detergents are inducers of enzyme activity. PMSF, a serine protease inhibitor, did not show any effect on the activity. The immobilized type II esterase was able to synthesize isoamyl acetate from isoamyl alcohol and p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. p-nitrophenyl acetate (acyl donor) in n-hexane. The resulting ester yield (42.8%), obtained at a low temperature (28°C) and with a very low amount of enzyme (4.6·10-5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes. synthesis for production purposes. synthesis for production purposes. -5 mg ml-1), indicates a high potential for type II esterase in isoamyl acetate synthesis for production purposes.