PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Clostridium lundense sp. nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen.
Autor/es:
CIRNE D; DELGADO O D; MARICHAMY S; MATTIASSON B
Revista:
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY.
Editorial:
The society for General Microbiology SGM
Referencias:
Lugar: Londres; Año: 2006 vol. 56 p. 625 - 628
ISSN:
1466-5026
Resumen:
strictly
anaerobic, mesophilic, endospore-forming, lipolytic bacterium, designated
strain R1T , was isolated from bovine rumen fluid and
characterized. Cells of this isolate were Gram-positive, non-motile rods that
formed spherical terminal spores. The overall biochemical and physiological characteristics
indicated that this strain should be placed in the genus Clostridium. The strain grew at
temperatures between 25 and 47° C (optimum, 37° C), at pH between 5 0 and 8 5 (optimum pH 5 57 0) and in NaCl concentrations of 03 % (w/v). The isolate was not able
to utilize glucose or other carbohydrates as carbon sources. The DNA G+C content was 31 2 mol%. Sequence analysis of the 16S rRNA gene of R1 T revealed that it has the closest match (98 % similarity)
with Clostridium
tetanomorphum DSM
4474 T . The highest levels of
DNADNA relatedness of the isolate were 61 9 and 54 3 %with Clostridium pascui DSM 10365 T and C. tetanomorphum DSM 4474 T , respectively. Based on 16S
rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNADNA hybridization data and distinct
phenotypic characteristics, strain R1 T (=DSM
17049 T =CCUG 50446 T ) was classified in the genus Clostridium, as a member of a novel
species, for which the name Clostridium lundense sp. nov. is proposed.