PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Study of the effect exerted by fructo-oligosaccharides from yacon (Smallanthus sonchifolius) root flour in an intestinal infection model with Salmonella Typhimurium
Autor/es:
VELEZ, E.M; CASTILLO N, A; MESON, O; GRAU, A; BIBAS BONET, M.E; PERDIGÓN G.
Revista:
BRITISH JOURNAL OF NUTRITION
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Lugar: Cambridge; Año: 2012 vol. 9 p. 1 - 9
ISSN:
0007-1145
Resumen:
Beneficial effects of prebiotics like inulin and fructo-oligosaccharides
(FOS) have been proven in health and nutrition. Yacon (Smallanthus
sonchifolius), an Andean crop, contains FOS (50-70 % of its dry weight)
and, therefore, is considered a prebiotic. Commercial FOS can
up-regulate total secretory IgA (S-IgA) in infant mice, prevent
infection with Salmonella in swine or enhance immune response for
Salmonella vaccine in a mouse model. Previously, we found that
administration of yacon root flour regulates gut microbiota balance and
has immunomodulatory effects without inflammatory responses. The aim of
the present paper is to analyse if yacon prevents enteric infection
caused by a strain of Salmonella enteritidis serovar Typhimurium (S.
Typhimurium) in a mouse model. BALB/c mice were supplemented with yacon
flour (45 d), challenged with S. Typhimurium and killed to study
pathogen translocation, total and specific IgA production by ELISA,
presence of IgA and other cytokines and Toll-like receptor 4 (TLR4) and
clustor of differentiation 206 (CD206) receptors positive cells by
immunofluorescence and histological changes. Yacon flour administration
had a protective effect from 15 to 30 d of treatment. We found a peak of
total S-IgA production without translocation of the pathogen for these
periods. At 30 d, there was an increase in IL-6 and macrophage
inflammatory proteins-1α+ cells and expression of the receptors CD206
and TLR4. Yacon flour did not have incidence in pathogen-specific S-IgA
production. Longer periods (45 d) of administration had no protective
effect. Therefore, yacon can prevent enteric infection caused by S.
Typhimurium when given up to 30 d; this effect would be mediated by
enhancing non-specific immunity, such as total S-IgA, that improves the
immunological intestinal barrier.