PROIMI 05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain
Autor/es:
RIVA MERCADAL JP; ISAAC P; SIÑERIZ F; FERRERO MA
Revista:
JOURNAL OF BASIC MICROBIOLOGY
Editorial:
Wiley-VCH
Referencias:
Año: 2010 p. 290 - 293
ISSN:
0233-111X
Resumen:
A technique developed to determine naphthalene
dioxygenase (NDO) activity was optimized and used to study the
biotransformation of indole to indigo by Pseudomonas sp. J26 whole
cells. The maximum production of indigo was achieved at 25 °C using 2.5
mM indole when J26 was grown in the complex medium JPP, while indole
concentrations higher than 4 mM proved toxic for cells. The maximum rate
of indigo production was 0.56 nmol min-1 mg dry biomass-1, obtaining
75.5 μiM of indigo after 8 h of incubation, while a maximal
concentration (138.1 μiM) of indigo was obtained after 20 h.
