PROIMI 05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
First report of linear megaplasmids in the genus Micrococcus
Autor/es:
DIB, JULIÁN RAFAEL; WAGENKNECHT, MARTIN; HILL, RUSSELL T.; FARÍAS, MARIA EUGENIA; MEINHARDT, FRIEDHELM
Revista:
PLASMID.
Editorial:
Elsevier
Referencias:
Año: 2010 vol. 63 p. 40 - 45
ISSN:
0147-619X
Resumen:
High-altitude wetlands (above 4,200 m) in the northwest of Argentina are considered pristine and extreme environments. Micrococcus sp. A1, H5, and V7, isolated from such environments, were shown to contain linear megaplasmids, designated pLMA1, pLMH5, and pLMV7, respectively. As known from linear plasmids of other actinomycetes, all three plasmids were resistant to λ exonuclease treatment, which is consistent with having terminal proteins covalently attached to their 5´ DNA ends. Electrophoretic mobility, Southern analysis, and restriction endonuclease patterns revealed pLMA1 and pLMH5 being indistinguishable plasmids, even though they were found in different strains isolated from two distant wetlands Laguna Azul and Laguna Huaca Huasi. Analysis of 16S rDNA sequences of Micrococcus sp. A1, H5, and V7 suggested a close relationship to Micrococcus luteus. Typing of isolates was performed using fingerprint patterns generated by BOX-PCR. Plasmid-deficient strains, generated from Micrococcus sp. A1, showed a significantly decreased resistance level for erythromycin.
