Optimization of chitosan-based films with vanillin for food packaging application

PONCE, A; TOMADONI B.; ANSORENA M.R.; M. PEREDA

Mar del Plata

Congreso; XVI Congreso Argentino de Ciencia y Tecnología de Alimentos CYTAL 2017; 2017

AATA

Recently, there has been a growing interest in the search for biodegradable and bio-based packaging materials due to the food safety and environmental problems causedusing the petrochemical-based plastics. Moreover, current research concerning supplementing natural antioxidants to enhance films functions includes the incorporation of essential oils or theaddition of phytochemicals due to their broad antimicrobial activities. Even though polyphenolic compounds have beenwidely applied in the food preservative field, their incorporation into chitosan filmsas a new and effective bioactive film needs to be further studied in terms of its structuralcharacterization and bioactivities. Hence, this work aims are to optimize the formulation of chitosan-based filmsadded with vanillin (a polyphenolic phytochemical) and to analyze its effect on the barrier and antioxidant properties of these films. Three-factor Box-Behnken design of response surface methodology (RSM) was used to optimize vanillincontent (0, 25 and 50% w/w chitosan), glycerol content (30, 45 and 60% w/w chitosan) and drying temperature (35, 50 and 65°C) for the development of active chitosan-based films with vanillin. Fifteen formulations were prepared according to the Box-Behnken design combining the different levels of the three factors. RSM and desirability function were used to simultaneously optimize the following response variables: polyphenol content (PC), antioxidant capacity (DPPH), total soluble matter (TSM) and water vapor permeability (WVP). CH film-forming solutions were prepared (2%, w/v) into 1% (v/v) acetic acid aqueous solution and glycerol (Gly) was added at different concentrations. Once the solution was homogeneous, vanillin (V) was incorporated as a natural cross-linking agent in different proportions and dissolved using a magnetic stirrer. The films were prepared by the casting technique in a convection oven at the corresponding drying temperatures. Predicted models were found to be significant (p