Frequently asked questions about chlorophyll fluorescence, the sequel

SCHANSKER G; CALATAYUD A; GUIDI L; KALAJI H M; LOSCIALE P; BRESTIC M; BUSSOTTI F; NEBAUER S G; FERRONI L; GOLTSEV V ; POLLASTRINI M; JAJOO A; YANNICCARI M; LI P; MISHRA V K; SAMBORSKA I A; MISRA A N; PANCALDI S; KUNDERLIKOVA K; PENELLA C; SURESH K; BąBA W; ZIVCAK M; TAMBUSSI E A; STIRBET A; CETNER M; OLSOVSKA K; SHELONZEK H; RUSINOWSKI S

PHOTOSYNTHESIS RESEARCH

SPRINGER

Lugar: Berlin; Año: 2016

0166-8595

Abstract Using chlorophyll (Chl) a fluorescence many aspects of the photosynthetic apparatus can be studied, both in vitro and, noninvasively, in vivo. Complementary techniques can help to interpret changes in the Chl a fluorescence kinetics. Kalaji et al. (Photosynth Res 122:121?158, 014a)addressed several questions about instruments, methods and applications based on Chl a fluorescence. Here, additional Chl a fluorescence-related topics are discussed again in a question and answer format. Examples are the effect of connectivity on photochemical quenching, the correction of FV/FM values for PSI fluorescence, the energy partitioning concept, the interpretation of the complementary area, robing the donor sideof PSII, the assignment of bands of 77 K fluorescence emission spectra to fluorescence emitters, the relationship between prompt and delayed fluorescence, potential problems when sampling tree canopies, the use of fluorescence parameters inQTL studies, the use of Chl a fluorescence in biosensor applications and the application of neural network approaches for the analysis of fluorescence measurements. The answersdraw on knowledge from different Chl a fluorescence analysis domains, yielding in several cases new insights.