INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Mapping resistance genes conferring tolerance to RWA (Diuraphis noxia) in barley (Hordeum vulgare).
Autor/es:
TOCHO ERICA; RICCI MÓNICA; TACALITI MARÍA SILVIA; GIMÉNEZ DANIEL; ACEVEDO ALBERTO; LOHWASSER U.; BÖRNER ANDREAS; CASTRO ANA MARÍA
Revista:
EUPHYTICA
Editorial:
SPRINGER
Referencias:
Lugar: Droschder; Año: 2012
ISSN:
0014-2336
Resumen:
The Russian wheat aphid (RWA) is one of
the most aggressive pests of barley and wheat. The
outbreak of RWA occurred in Argentina in 2008
caused serious damage to barley cultivars. The most
effective and sustainable method of RWA control is to
identify new resistance genes. The purpose of the
current research was to map RWA resistance genes in
a set of double haploid (DH) lines of the Oregon-
Wolfe Barley (OWB) mapping population derived
from the cross between OWBDOM and OWBREC. The
DH and both parental lines were screened for antixenosis,
tolerance and antibiosis to RWA. There was
significant variation among the DH lines in most of the
traits studied. However, only tolerance resulted in
significant quantitative trait loci (QTLs) associated
with the molecular markers. Two main QTLs were
identified. These explained 90 and 79 % of the
variability of foliar area and chlorophyll content,
respectively, of infested and control plants. The initial
and final foliar area and the variation in foliar area
were associated with the same molecular markers on
chromosome 2H (BmAc0125, Vrs1, BmAc0144f and
DH and both parental lines were screened for antixenosis,
tolerance and antibiosis to RWA. There was
significant variation among the DH lines in most of the
traits studied. However, only tolerance resulted in
significant quantitative trait loci (QTLs) associated
with the molecular markers. Two main QTLs were
identified. These explained 90 and 79 % of the
variability of foliar area and chlorophyll content,
respectively, of infested and control plants. The initial
and final foliar area and the variation in foliar area
were associated with the same molecular markers on
chromosome 2H (BmAc0125, Vrs1, BmAc0144f and
DOM and OWBREC. The
DH and both parental lines were screened for antixenosis,
tolerance and antibiosis to RWA. There was
significant variation among the DH lines in most of the
traits studied. However, only tolerance resulted in
significant quantitative trait loci (QTLs) associated
with the molecular markers. Two main QTLs were
identified. These explained 90 and 79 % of the
variability of foliar area and chlorophyll content,
respectively, of infested and control plants. The initial
and final foliar area and the variation in foliar area
were associated with the same molecular markers on
chromosome 2H (BmAc0125, Vrs1, BmAc0144f andBmAc0125, Vrs1, BmAc0144f and
BmAg0113e). The positive alleles were provided by
OWBDOM. The content of chlorophyll was associated
with the marker loci WMC1E8, MWG912, ABC261,
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
with the marker loci WMC1E8, MWG912, ABC261,
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
OWBDOM. The content of chlorophyll was associated
with the marker loci WMC1E8, MWG912, ABC261,
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
with the marker loci WMC1E8, MWG912, ABC261,
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
). The positive alleles were provided by
OWBDOM. The content of chlorophyll was associated
with the marker loci WMC1E8, MWG912, ABC261,
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
with the marker loci WMC1E8, MWG912, ABC261,
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
DOM. The content of chlorophyll was associated
with the marker loci WMC1E8, MWG912, ABC261,
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
WMC1E8, MWG912, ABC261,
MWG2028 and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
and Blp on chromosome 1H, with the
positive alleles provided by OWBREC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
REC. Both parents
contributed to different tolerance traits, with foliar
area and chlorophyll content remaining as the plant
traits most affected by aphid feeding. The QTLs found
in this population are new RWA resistance loci. A
sequence homology search was performed to derive
the putative function of the genes linked to the QTLs.
Keywords Molecular markers RWA Barley
Tolerance Candidate genes Quantitative trait locus
Tolerance Candidate genes Quantitative trait locus
Molecular markers RWA Barley
Tolerance Candidate genes Quantitative trait locus
Candidate genes Quantitative trait locus