IQUIR   05412
INSTITUTO DE QUIMICA ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Standard addition analysis of fluoroquinolones in human serum in the presence of the interferent salicylate using lanthanide-sensitized excitation-time decay luminescence data, multivariate curve resolution
Autor/es:
V.A. LOZANO, R. TAULER, G.A. IBAÑEZ, A.C. OLIVIERI,
Revista:
TALANTA
Editorial:
Elsevier
Referencias:
Año: 2009 vol. 77 p. 1715 - 1723
ISSN:
0039-9140
Resumen:
Three fluoroquinolone antibiotics (ciprofloxacin, norfloxacin and danofloxacin) have been determined in
human serum in the presence of the potential interferent salicylate, by processing lanthanide-sensitized
excitation-time decay matrix data for their terbium (III) complexes. The algorithm employed, multivariate
curve resolution-alternating least-squares, is one of the fewmethodologies which permit the achievement
of the second-order advantage in the presence of a high degree of overlapping between the time decay
profiles for the analyte and the interferent complexes. Furthermore, the presence of analytebackground
interactions makes it necessary to employ the standard addition method for successful quantitation.
Both simulations and experiments showed that the modified standard addition method was suitable for
this purpose, in which the test data matrix was subtracted from the standard addition matrices, and
quantitation proceeded using classical external calibration procedure. The analyte concentration ranges
were all within the therapeutic range, i.e., 06 mg/L in serum, with final concentrations in the measuring cell in the order of 0.2 mg/L.