IQUIR 05412
INSTITUTO DE QUIMICA ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Desing and Development of a Receptor Assay Based on the Inhibition of Carboxypeptidase Activity Coupled with and Electrochemical D-Alanine sensor for the Detection of β-Lactamic Antibiotics
Autor/es:
ALARCÓN, S. H.; RIVAS, G.; FEO MANGA, J.; COMPAGNONE, D.; PEPE, A.
Revista:
Sensor and Microsystems
Editorial:
World Scientific Publishing Co. Pte. Ltd
Referencias:
Año: 2009 p. 20 - 25
ISSN:
0254-4725
Resumen:
A microbial receptor protein with carboxypeptidase activity and a D-Alanine sensor wereused to design an inhibition assay with electrochemical detectiton. This assay has beendevcloped for thè quantitative determination of p-lactam. Optimisation of thè inhibitionassay conditions allowed us to realise an assay with comparable analytical behaviour thanthè classica! microbiological and other assays. The assay give a detection limit of 1ng.mL"1.
