COMPARISON OF THE PREDICTIVE ABILITY OF SEVERAL SECOND ORDER MULTIVARIATE METHODS IN THE SIMULTANEOUS DETERMINATION OF TWO THERAPEUTIC DRUGS IN HUMAN URINE

HURTADO SANCHEZ, M.C.; DURAN MERAS, I.; RODRIGUEZ CACERES, M. I.; JIMENEZ GIRON, A.; OLIVIERI, A. C.

TALANTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 vol. 88 p. 609 - 616

0039-9140

The aim of this paper is to study the applicability of second-order multivariate methods inthe simultaneous determination of two therapeutic drugs in human urine samples. Thestudied drugs, irinotecan and thalidomide, are used in the treatment of malignant tumours.Irinotecan (CPT-11) is used to treat colon cancer; recent studies have shown the benefits ofusing thalidomide in combination with CPT-11 in the treatment of this disease. CPT-11 ishighly fluorescent, but the native fluorescence of thalidomide is very weak. The second18order methods assayed were parallel factor analysis (PARAFAC), unfolded partial least19squares (U-PLS) and multidimensional partial least-squares (N-PLS), both combined withthe residual bilinearization procedure (RBL). The excitation-emission matrices (EEMs) ofthe samples were recorded as analytical signal. The accuracy and precision of thealgorithms were evaluated through the root mean square error of prediction (RMSEP) andthe elliptical joint confidence region test (EJCR), obtaining better results with PARAFAC,which was successfully applied to the determination of thalidomide and CPT-11 in humanurine samples, after a previous liquid-liquid extraction with chloroform.