EFFECT OF GENISTEIN ON PARAQUAT-INDUCED HEPATOTOXICITY IN RATS

SEMENIUK M; QUINTANA A; RUIZ ML; CERÉ L; RIGALLI JP; CIRIACI N; CATANIA VA

Buenos Aires

Congreso; Reunión conjunta de Biociencias; 2017

SAIC-SAFIS-SAFE-etc.

Background and aims: Previously we found that genistein (GNT) treatment induced P-glycoprotein (P-gp) expression and activity in rat liver. The aim of this study was to evaluate if GNT treatment reduce hepatotoxicity induced by the herbicide paraquat (PQ), a known P-gp substrate.Methods: Male Wistar rats were randomized in 4 experimental groups (n=3). Control, GNT (5 mg/Kg/day s.c.,4 days), PQ (50 mg/Kg/day i.p., last day) and GNT+PQ (PQ injected the last day of GNT treatment). On the 5th day hepatic lipoperoxidation (LPO) was evaluated by the thiobarbituric acid reactive substances (TBARS) methodology. Serum levels of alanine transaminase (ALT) and aspartate transaminase (AST) were measured with commercial kits. Liver samples were processed for histological analysis. Hepatic glutathione-S-transferase (GST) activity, which is associated with the prevention of PQ toxicity, was determined by UV-spectrophotometry.Results (% of control): Pretreatment of PQ-intoxicated rats with GNT reduced hepatic LPO as compared with PQ alone (Control: 100 ± 2, GNT: 99 ± 12, PQ: 120 ± 5 *, GNT+PQ: 101 ± 6) (* p