GERANIOL (GO) PREVENTS INTESTINAL MRP2 DOWN-REGULATION IN RATS WITH FRUCTOSE-INDUCED METABOLIC SYNDROME (MS)

ZECCHINATI F; TOCCHETTI GN; CILENZO A; VILLANUEVA SSM; LONDERO AS; PERDOMO VG; MOTTINO A; ARANA MR; BARRANCO MM; GARCÍA F

Mar del Plata

Congreso; ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC) LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) XLVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE) VII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE NANOMEDICINA (NANOM; 2016

SAIC, SAFE, NANOMEDAR, AACYTAL.

Intestinal Mrp2 is an ABC transporter that limits the absorptionof xenobiotics orally ingested, thus acting as a biochemical barrier./S is a pathological condition characterized by insulin resistance,hyperinsulinemia and dyslipidemia as Yell as by chronic inflammationand oxidative stress 1S. +n previous studies Ye observedthat /SliMe conditions induced by fructose in drinMing Yater v/v, during YeeMs F47, reduced the expression and activity ofintestinal /rp in rats. 9e here evaluated the effect of )1 mg/Mg/day p.o, a monoterpene Yith antioxidant and antiinflammatoryproperties, in preventing fructoseinduced /rp alterations.After YeeMs of cotreatment, )1 prevented doYnregulation of/rp protein evoMed by /S, as detected by Yestern blotting C, F47 , F47)1 , 2.. /rp activity Yasevaluated using the in vitro model of everted intestinal sacs. Effluxof the /rp substrate &02S) Yas decreased in F47 rats respect to C (2., Yhereas /rp activity in the cotreatedgroup remained unchanged. Additionally, fructose generated 1Sin intestinal tissue as indicated by increasing lipid peroxidationproducts , 2. and activity of the antioxidant enzymeS1& , 2., respect to C, and by decreasing )SH/)SS) ratio respect to C 2.. Additionally, fructoseincreased the intestinal level of the proinflammatory cytoMinesIL-1E , 2. and +L , 2. respect to C. +n 2. and +L , 2. respect to C. +nterestingly,)1 not only reversed the parameters of S/ studied,but also normalized the intestinal index of oxidative stress andIL-1 E and +L levels in the F47 group. Administration of )1alone did not affect the parameters studied. Conclusion the dataindicate that 1S and inflammation could be important mediatorsof /rp doYnregulation under /SliMe conditions, and that )1could be a potential therapeutic tool to prevent the impairment inthe intestinal barrier by counteracting the effect of such mediators.