24. EFFECT OF INCREASING DOSES OF PROANTHOCYANIDIN EXTRACTED FROM Ligaria cuneifolia ON BLOOD FLUIDITY IN RATS FED CHOLESTEROL-ENRICHED DIET

GONZALVÉS J,; GALLIANO S; DOMINIGHINI A,; CROSSETTI D,; FRANCES D.E; RONCO M,; MONTI J; WAGNER M,; CARNOVALE C.; LUQUITA A

Congreso; Sociedad de Biología Rosario, XIV Congress and XXXII Annual Meeting; 2013

Cuneifolia Ligaria (Lc) is an argentine hemiparasitic plant used in folk medicine for decrease of blood fl uidity diminishing cholesterol (Co). Our aim was analyzes the effect of increasing doses of the PLc extract on the blood fl uidity of rats fed a diet enriched in Co. Adult male Wistar rats (n = 24) were fed for 28 days with ?standard diet ? adding with Co (97% purity) 8 g / kg of diet and 28% corn oil (wt / wt of diet). The group C (Control, n = 6) received saline and group T (Treated) received the PLc extract, both groups in an ip injection every 24 hours, 3 consecutive days. The doses used were 1.5 mg/100 g body weight (BW) (T1, 5) (n = 6), 3 mg/100 g PC (T3) (n = 6) and 6 mg/100 g PC (T6) (n = 6). The day of the experiment, 24 hours after the last injection, the animals were anesthetized by ip with sodium pentobarbital (50 mg / kg BW); the blood was obtained by cardiac puncture. Total Co (mg%): C: 108.54±2.21; T1,5: 71.56±3.64*; T3: 61.18±3.30*; T6: 67.66±1.17*; CoLDL (mg%): C: 27.76±1.54; T1,5: 13.59±1.08*; T3: 16.13±1.33*; T6: 18.00±0.39*; CoHDL (mg%): C: 25.00±0.74; T1,5: 22.10±2.37*; T3: 15.57±0.60*; T6: 18.75±0.95* (*p