EFFECT OF THE COMBINED TREATMENT OF INTERFERON ΑLFA-2B (IFN) AND VITAMIN E (VITE) ON THE DEVELOPMENT OF LIVER CANCER

COMANZO C; FERRETTI A; QUIROGA, ARIEL D.; LUCCI A; LORENZETTI F; CEBALLOS MP; CARRILLO, MARÍA C.; VERA M; ABBONDANZIERI A; ALVAREZ, MARÍA DE L.

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2017 vol. 77 p. 571 - 571

0025-7680

IFN has been used for the treatment of patients with hepatitis Bor C, and VitE was shown to have inhibitory effects on liver cancerdue to its antiangiogenic, antioxidant and antiproliferative activities.We aimed to evaluate if the combined therapy of IFN withVitE has a synergistic inhibitory effect on liver cancer development,cell proliferation and apoptosis compared to each separate therapy.Adult male Wistar rats were subjected to a two-phase model ofliver cancer (initiated-promoted, IP group). Initiation: 2 i.p. doses ofdiethylnitrosamine (150 mg/kg bw) 2 weeks apart. One week afterthe last injection, rats received 20 mg/kg bw of 2-acetylaminofluoreneby gastric probe 4 days per week for 3 weeks. IP rats alsoreceived IFN 6,5x105 U/kg bw (IFN group); α-Tocopherol, an isomerof VitE, 3 mg/kg bw (E group); and both drugs in a combined therapy(IFN-E group). After treatment and euthanasia, the livers wereremoved and altered hepatic foci (AHF) were determined by immunohistochemistry(rGST-P). To determine fibrosis, collagen was analyzedby Direct Red 80 staining. Also, we analyzed oxidative stress(OS) by TBARS, and proliferation (proliferating cell nuclear antigen,PCNA) and apoptosis (cit. c release) by immunolotting. Results:IFN-group: apoptosis (+162%*), OS (+75%*), PCNA protein levels(-68%*), number of AHF per liver (-35%*), percentage of liver occupiedby foci (-83%*) and percentage of fibrosis (-39%*). E-group:apoptosis (+127%*), OS (-40%*), with no changes in PCNA, in thenumber and percentage of AHF and fibrosis. IFN-E group: apoptosis(-60%#), OS (-120%#), PCNA (+102%#), number and percentageof AHF (+35%# and +80%#) and percentage of fibrosis (+29%#)(*p