Hepatic Gene Transfer of Human Aquaporin-1 Improves Bile Salt Secretory Failure in Rats with Estrogen-Induced Cholestasis

DANIELLI M; MARINELLI RA; DANIELLI M; MARINELLI RA; MARRONE J; LEHMAN GL; MARRONE J; LEHMAN GL; SORIA LR; LAROCCA MC; SORIA LR; LAROCCA MC

HEPATOLOGY (BALTIMORE, MD.)

JOHN WILEY & SONS INC

Año: 2016

0270-9139

The adenoviral gene transfer of human aquaporin-1 (hAQP1) water channels to the liver of ethinylestradiol (EE)-induced cholestatic rats improves bile flow, in part by enhancing canalicular hAQP1-mediated osmotic water secretion. To get further insight into the mechanisms of EE-cholestasis improvement, we now studied the biliary output of bile salts (BS) and the functional expression of canalicular BS export pump (BSEP; ABCB11). Adenovector encoding hAQP1 (AdhAQP1) or control vector was administered by retrograde intrabiliary infusion. AdhAQP1-transduced cholestatic rats increased the biliary outputs of major endogenous BS (50-80%, P