IFISE   05411
INSTITUTO DE FISIOLOGIA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Chymotrypsinpoly vinyl sulfonate interaction studied by dynamic light scattering and turbidimetric approaches Biochimica et Biophysica Acta
Autor/es:
VALERIA BOERIS; SPELZINI, D.; PELLETEIRO SALGADO, J; PICO GUILLERMO; ROMANINI, D; FARRUGGIA, B
Revista:
Biochimica Biophysic Acta
Editorial:
Elsevier
Referencias:
Lugar: Anstedand; Año: 2008 vol. 1780 p. 1032 - 1037
Resumen:
The formation of non-soluble complexes between a positively charged protein and a strong anionic
polyelectrolyte, chymotrypsin, and poly vinyl sulfonate, respectively, was studied under different
experimental conditions such as pH (13.5), protein concentration, temperature, ionic strength, and the
presence of anions that modifies the water structure. Turbidimetric titration and dynamic light scattering
approaches were used as study methods. When low proteinpolyelectrolyte ratio was used, the formation of
a soluble complex was observed. The increase in poly vinyl sulfonate concentration produced the interaction
between the soluble complex particules, thus inducing macro-aggregate formation and precipitation.
Stoichiometry ratios of 500 to 780 protein molecules were found in the precipitate per polyelectrolyte
molecule when the medium pH varied from 1.0 to 3.5. The kinetic of the aggregation process showed to be of3.5), protein concentration, temperature, ionic strength, and the
presence of anions that modifies the water structure. Turbidimetric titration and dynamic light scattering
approaches were used as study methods. When low proteinpolyelectrolyte ratio was used, the formation of
a soluble complex was observed. The increase in poly vinyl sulfonate concentration produced the interaction
between the soluble complex particules, thus inducing macro-aggregate formation and precipitation.
Stoichiometry ratios of 500 to 780 protein molecules were found in the precipitate per polyelectrolyte
molecule when the medium pH varied from 1.0 to 3.5. The kinetic of the aggregation process showed to be offies the water structure. Turbidimetric titration and dynamic light scattering
approaches were used as study methods. When low proteinpolyelectrolyte ratio was used, the formation of
a soluble complex was observed. The increase in poly vinyl sulfonate concentration produced the interaction
between the soluble complex particules, thus inducing macro-aggregate formation and precipitation.
Stoichiometry ratios of 500 to 780 protein molecules were found in the precipitate per polyelectrolyte
molecule when the medium pH varied from 1.0 to 3.5. The kinetic of the aggregation process showed to be ofpolyelectrolyte ratio was used, the formation of
a soluble complex was observed. The increase in poly vinyl sulfonate concentration produced the interaction
between the soluble complex particules, thus inducing macro-aggregate formation and precipitation.
Stoichiometry ratios of 500 to 780 protein molecules were found in the precipitate per polyelectrolyte
molecule when the medium pH varied from 1.0 to 3.5. The kinetic of the aggregation process showed to be of
first order with a low activation energy value of 4.2±0.2 kcal/mol. Electrostatic forces were found in the
primary formation of the soluble complex, while the formation of the insoluble macro aggregate was a
process driven by the disorder of the ordered water around the hydrophobic chain of the polymer.rst order with a low activation energy value of 4.2±0.2 kcal/mol. Electrostatic forces were found in the
primary formation of the soluble complex, while the formation of the insoluble macro aggregate was a
process driven by the disorder of the ordered water around the hydrophobic chain of the polymer.