Carboxylated Latex Particles Sensitized with Antigenic Proteins of various Diseases for their Use as Immunoagglutination Reagents

GONZALEZ, VERÓNICA D.G.; GARCÍA, VALERIA S.; PERETTI, LEANDRO E.; PROCHETTO, SANTIAGO D.; GUGLIOTTA, LUIS M.

Florianópolis - SC

Congreso; 12º Congresso Brasileiro de Polímeros (12º CBPol); 2013

Monodisperse polymer colloids with carboxyl functionality were synthesized through a two-step emulsion polymerization process. The synthesized ?core-shell? particles were used as support for their sensitization (by covalent coupling) with antigens of Trypanosoma cruzi, Toxoplasmas gondii and Leptospira interrogans. The resulting latex-protein complexes were used in immunoagglutination assays for detecting Chagas disease, Toxoplasmosis and Leptospirosis, respectively. In all cases, high fractions of proteins chemically-bound to carboxyl groups were observed and all latex-protein complex allowed discrimination between sera from infected and non-infected patients