Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice

VERÓNICA GONZALEZ; ANA MARÍA GENNARO; IVANA GABRIELA REIDEL; ANTONELLA GIORELLO; CAROLINA VEAUTE; MARÍA INÉS GARCÍA; LUIS FERNANDO CALVINHO

International Journal For Research In Applied And Natural Science

International Journal for Research

Año: 2017 vol. 3

The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection method. Immune response was assessed by immunization of Balb/c mice with: Lip+BSA, Lip+BSA+CpG, CpG+BSA or aluminium hydroxide (Al(OH)3+BSA). Liposomal formulations were able to induce high antibody levels. Lip+BSA+CpG led to higher IgG levels than Lip+BSA (p