INTEC   05402
INSTITUTO DE DESARROLLO TECNOLOGICO PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
SIMULATION OF 1D AND 2D ELECTROPHORETIC SEPARATIONS IN MICROFLUIDICS CHIPS
Autor/es:
PABLO A. KLER, FABIO A. GUARNIERI AND CLAUDIO L. A. BERLI
Revista:
MECANICA COMPUTACIONAL
Editorial:
AMCA
Referencias:
Lugar: Tandil; Año: 2009 vol. 28 p. 2053 - 2070
ISSN:
1666-6070
Resumen:
Electrophoretic separations comprise a group of analytical techniques such as capillary zone
electrophoresis (CZE), isoelectric focusing (IEF), isotachophoresis (ITP) and free flow electrophoresis
(FFE). In all cases, separation is based on the dissimilar mobility of ionic species under the action of an
external electric field. These techniques, which are widely used in chemical and biochemical analysis,
have been miniaturized in the last years and now represent one of the most important applications of
the lab-on-a-chip technology. In a previous work, a generalized numerical model of electrophoresis on
microfluidic devices was presented. The model is based on the set of equations that governs electrical
phenomena (Poisson equation), fluid dynamics (Navier-Stokes equations), mass transport (Nerst-Planck
equation) and chemical reactions. Also the relationship between the buffer characteristics (ionic strength,
pH) and surface potential of channel walls is taken into consideration. In this work, three application
examples are presented: (a) an IEF assay with immobilized pH gradient (IPG) including the influence
of electro-osmotic flow on its performance, (b) an IEF assay involving ampholyte-based pH gradient,
and (c) a 2D electrophoresis, involving FFIEF plus CZE. The numerical simulation is carried out by
using PETSc-FEM (Portable Extensible Toolkit for Scientific Computation - Finite Elements Method),
in a Python environment developed at CIMEC using high performance parallel computing and solving
techniques based on domain decomposition methods.