INTEC   05402
INSTITUTO DE DESARROLLO TECNOLOGICO PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
Nitrogen metabolism is related to improved water-use efficiency of nodulated alfalfa grown with sewage sludge under drought
Autor/es:
MARÍA LAURA FIASCONARO, MANUEL SÁNCHEZ-DÍAZ, MA CARMEN ANTOLÍN
Revista:
JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE-ZEITSCHRIFT FUR PFLANZENERNAHRUNG UND BODENKUNDE
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2013 vol. 176 p. 110 - 117
ISSN:
1436-8730
Resumen:
Leguminous plants grown in sewage sludge?amended soils can acquire nitrogen by assimilation
of nitrate and ammonium from the soil solution or from atmospheric-dinitrogen (N2) fixation
through association with N2-fixing bacteria. We proposed that operation of both metabolic processes
could contribute to alleviate the impact of drought in sludge-treated plants. A greenhouse
experiment was conducted to evaluate the involvement of nodule metabolism in the use efficiency
of water and N in sludge-treated plants. Treatments comprised (1) plants inoculated with
rhizobia and amended with sewage sludge; (2) plants inoculated with rhizobia without any
amendment; and (3) noninoculated plants supplied with ammonium nitrate, each under wellwatered
and drought conditions. Under drought, sludge-treated plants had increased plant
growth and higher photosynthetic and water-use efficiencies than untreated plants. Drought stimulated
nitrate reductase and GS/GOGAT activities but did not affect the activities of phosphoenolpyruvate
carboxylase and malate dehydrogenase or the leghemoglobin concentration. The
results suggest that under drought conditions, both N2 fixation and nitrate assimilation in nodules
of sludge-treated plants contributed to improve plant N supply and to increase the drought tolerance
of alfalfa.