Synthesis of Latex-Antigen Complexes from Single and Multiepitope Recombinant Proteins. Application in Immunoagglutination Assays for the Diagnosis of Trypanosoma cruzi Infection

GARCIA, V.S.; GONZALEZ, V.D.G.; CAUDANA, P.C.; VEGA, J.R.; MARCIPAR, I.S.; GUGLIOTTA, L.M.

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2011

0927-7765

This article investigates the physical adsorption and the chemical coupling of three different recombinant proteins of Trypanosoma cruzi, not previously investigated for developing agglutination tests, onto sub-micrometric core-shell carboxylated particles to be used in an immunoassay for detecting the Chagas disease. The coupled recombinant proteins were the multiepitope protein CP2 (derived from three antigenic peptides) and two single proteins (RP1 and RP5). In all cases, high fractions of proteins chemically-bound to carboxyl groups were observed and the highest surface densities of linked proteins were attained at pH values close to the proteins isoelectric point. The latex-CP2 complex obtained by covalent coupling produced better results than both the latex-RP1 and the latex-RP5 complexes in the immunoagglutination assays, i.e., higher discrimination between sera from a panel of 16 infected and 16 non-infected patients.