Informe Finalización de proyecto "Nanosized Delivery of Radiopharmaceuticals"

C. FLORES; E. ACHILLI; M. GRASSELLI; SILVIA DEL VALLE ALONSO

2014-04-01/2019-03-01

Nanotecnología y Nanobiotecnología

Salud humana

Nanomedicines, especially in round shape particles or nanoparticles (NPs), are studied for application in the field of diagnosis and treatment of cancer. It is expected that this novel nanomaterials will have an improved degree of specificity to tumour cells through interaction with cell surface receptors. Noble metal nanoparticles have high biology oriented applications,especially the use of gold nanoparticles for their proprietary stabilising conditions for use applications requiring harsher conditions. In this report it is described as a novel hybrid NPs (HNPs), containing a gold core and a multilayer of albumin stabilised by the radiation-inducedcrosslinking method. HNPs are decorated with a DOTA-Bombesin synthetic peptide in orderto address cancer cells which overexpress GRP receptors. Flow cytometry analysis and confocal microscopy were applied for study the in-vitro specific interaction of these nanoconstructs to PC-3 and NCI-H460 cell lines. It was demonstrated that the peptide decoration is required for the HNP uptake and that the internalisation process is energy dependent.This final report is available on the IAEA website. In it I am included as a participant and it is detailed that my thesis was carried out within the framework of this project.