UNDESIRABLE LITTER?S METABOLIC CONSEQUENCES DUE TO MATERNAL MALNUTRITION THROUGHOUT PREGNANCY

GIORDANO A; SPINEDI E; ALZAMENDI A; HARNICHAR A E; GIOVAMBATTISTA A; MIGUEL I; REY A

Modalidad Virtual

Congreso; Reunión Anual de Sociedades de Biociencia SAIC SAI SAFIS; 2020

Sociedad Argentina de Investigación Clínica

It is widely accepted that diet quality supply during gestation programs pup´s development. Our aim was to evaluate the impact of fructose rich diet (FRD) intake in adult females whose mothers consumed FRD during pregnancy. For this purpose on pregnancy day 1, dams were provided either with tap water alone or containing fructose (10% w/v; FRD) and fed ad libitum with chow up to delivery. Weaned female pups were maintained until 60-days old with normal diet ad libitum. Randomly, one group of pups was sacrificed (C and F considering maternal diet) and the remaining were split into two subgroups: one received normal diet (CC and FC) and the other FRD (CF and FF) for three weeks. Body weight (BW) and caloric intake (CI) were recorded from day 21 until 81 of age, data were analyzed by split plot ANOVA. At both sacrifice days (60 and 81) blood was collected and retroperitoneal adipose tissue (RPAT) was excised and weighed. T-tests were performed for 60 day´s results and 2-way ANOVA for 81 ones (programming and diet as variables). At age 60 days, F females only showed differences in CI in an age x programming dependent manner, with lower CI than C rats as age increases, but no metabolic changes were seen. At 81 days, all groups increased their BW in an age dependent manner, but programming and adult diet increased it even more. CI increased by FRD, showing FF rats the highest CI among groups. While plasma glucose levels did not vary, insulin increased by adult FRD. Triglycerides levels in FF animals were the highest, over all other groups. Moreover, programming by FRD induced higher levels of leptin in line with higher RPAT mass. In conclusion, FRD intake during pregnancy induces a programming effect on female litters, installed at age 81 days. Indeed, because maternal FRD intake effect appeared, the unbalanced diet consumption seems to trigger a metabolic misprogramming in adulthood. This effect could probably be due to the in utero high FRD pups? exposure. PICT 2017-2334