INTERACTIONS BETWEEN HYBRID NANOPARTICLES DESIGNED BY RADIO-SYNTHESIS AND THE HEMATOLOGY SYSTEM

M. GRASSELLI; C. Y. FLORES; A. CASTELLO; E. ACHILLI

Mar del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

SAIC, SAFE, SAP, SAB, AACYTAL, NANOMED-ar, HCS

Nanoparticles (NPs) are extremely promising due to their physicochemical properties and their distinctive features for therapeutic applications. When NPs enter the bloodstream, they immediately interact with plasma and hematology cells (erythrocytes, monocytes and platelets). In the case of blood plasma, the surface of the NPs interacts with several different biomolecules, mostly proteins, forming irreversible layers called the ´protein corona´. This adsorption of proteins onto NPs modify the diverse physicochemical properties of NPs such as size, surface charge, surface composition, and functionality, hence giving NPs a new biological identity and different biological responses.The aim of this work is to determine the effect of hybrid NPs on the formation of the protein corona in vitro and erythrocyte interaction and coagulation time. These hybrid NPs are AuNPs coated with human serum albumin (Alb) multilayers by a novel radiation-induced crosslinking process and are called Au/Alb core/shell nanoparticles (Au/Alb NPs). Albumins from human serum were added to the AuNPs suspension, 30% V/V ethanol was added and then these NPs were irradiated at 10 kGy with a gamma source to induce protein crosslinking. We determined the hemolytic properties of NPs through quantitative colorimetric determination of hemoglobin in whole blood by cyanmethemoglobin and coagulation assays following the specialized protocol. The protein corona effect was evaluated in terms of the hydrodynamic diameter (HD) of the NPs, isolation of the corona complex by size exclusion chromatography and polyacrylamide gel electrophoresis. Hybrid NPs did not present negative effects on the coagulation time nor appreciable hemolytic activity at the concentrations tested. In addition not changes in the HD were found. In conclusion, our results suggest that Au/Alb NPs show a better hemocompatibility than albumin monolayer and pegilated Au-NPs.