Polymeric liposomes with phospholipids from lung surfactant as a potential delivery system for pulmonary tissue.

CHIARAMONI, NADIA; BUJAN, ARIANA; ALONSO, SILVIA DEL VALLE

San Luis

Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Biofísica; 2019

Sociedad Argentina de Biofísica

Liposomes have been widely used for many applications. In the last decades, polymeric lipids have been studied as an option to increase liposome stability. (Temprana et al. 2012).In this work we used 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC), a photopolymerizable lipid. When this diacetylenic lipid is in a molar ratio of 1:1 with DMPC, it forms polymers upon UV irradiation, increasing liposome stability (Chiaramoni et al., 2010). Lung surfactant is a complex with the specific function of reduces surface tension at the pulmonary air-liquid interface. Regarding its composition, about 80% and 10% of phospholipids are PC and PG, respectively, with minor contributions of PE and others. PC species are distributed in PC16:0/16:0 (DPPC), PC16:0/16:1 (POPC) and PC16:0/14:0 (PMPC) (Bernhard, 2016).In this work, we combined lipids to form polymers but with de addition of DPPC and PMPC in order to direct these formulations to lung tissue. The hypothesis is that the presence of lipids that belong to surfactant will improve polymer interaction with pulmonary tissue. Several formulations were obtained by combining DC8,9PC, DMPC, DPPC and PMPC. Afterwards, they were polymerised by UV irradiation, and polymerisation efficiency was determined in order to select those formulations that were more efficient.For characterisation, particle size was determined by DLS and morphology by TEM. Membrane hydrophobicity was studied with the probe merocyanine 540. Cell viability was also assessed by the MTT method.We conclude that those formulations with higher amount of DPPC instead of PMPC presented a more rigid surface, giving as a result enhanced polymerisation. None of the formulations presented toxic effects in cell cultureThese formulations will be combined with mucolytic molecules for further assays. ReferencesBernhard, W. (2016). Lung surfactant: Function and composition in the context of development and respiratory physiology. Annals of Anatomy 208: 146?150.Chiaramoni, N.S., Gasparri, J., Speroni, L., Taira, M.C., Alonso, S. del V. (2010). Biodistribution of liposome/DNA systems after subcutaneous and intraperitoneal inoculation. J. Liposome. Res. 20: 191-201Temprana, C.F., Duarte, E.L., Femia, A.L., Alonso, S. del V., Lamy, M.T. (2012). Structural effect of cationic amphiphiles in diacetylenic photopolymerizable membranes. Chem. Phys. Lipids. 165: 589-600.