TESTOSTERONE EFFECTS ON THE THERMOGENIC PROGRAM OF THE INGUINAL ADIPOSE TISSUE FROM THE ADULT RAT

SPINEDI E; ZUBIRÍA, MARÍA GUILLERMINA; GIOVAMBATTISTA, ANDRÉS; HARNICHAR, EA

Congreso; Reunión conjunta de Sociedades de Biociencias.; 2017

Little is known regarding the effects of androgens on the thermogenicprocess of beige adipocytes. We previously demonstratedan inhibitory action of Testosterone (T), in vivo and in vitro, in thethermogenic process of retroperitoneal adipose tissue (AT) cells. Inthe present study we evaluated the effect of T on the thermogenicactivity of the subcutaneous AT (Inguinal AT: IAT). For this aim, threegroups of male S-D rats were used: control (CTR), pre-pubertally (27day-old) orchidectomized (ODX) and control pair-fed (CTR-PF). At60 days of age, the IAT pad was dissected and adipocyte precursorcells (APCs) were isolated. APCs were cultured and differentiated (3days) with a classic pro-beige cocktail. On differentiation day (Dd)8, cells were processed to quantify UCP-1 gene expression (qPCR).Additionally, APCs from IAT from adult CTR rats were isolated anddifferentiated with the classic pro-beige cocktail in the absence orpresence of 0.1 μM T (basal: B or T, respectively), thereafter cellsremained in culture without or with T. On Dd 8, 10 μM FSK was addedfor 4 h to subsets of B and T cells: B without FSK (B-B), B withFSK (B-FSK), T without FSK (T-B) and T with FSK (T-FSK). Cellswere then processed to quantify UCP-1 mRNA levels. APCs fromODX animals differentiated in vitro showed a higher UCP-1 mRNAexpression (p