AN ALBUMIN ? BASED GAMMA IRRADIATED NANOPARTICLE AS A POTENTIAL NANOVEHICLE FOR DRUG DELIVERY SYSTEMS: BIOPHYSICAL CHARACTERISATION AND TOXICITY IN ZEBRAFISH

JIMENA PRIETO; VINCENT RAUSSENS; MACARENA SIRI; SILVIA DEL VALLE ALONSO; JEAN-MARIE RUYSSCHAERT; DANIELA IGARTUA; MARIANO GRASSELLI; FERNANDO ALVIRA; ESTEFANÍA ACHILLI

Santos, SP

Congreso; XLII Congreso de la Sociedad Brasilera de Biofísica; 2017

Sociedad Brasilera de Biofisica

A bovine serum albumin-based nanoparticle (BSA NP) was achieved by γ-irradiation of a protein ethanol solution. This study centres on the biophysicalcharacterisation of the nanoparticle. Confirmation of the NP as an entity wasstated by D.L.S and microscopic techniques (T.E.M, S.E.M. and A.F.M.).Preservation of the main structural features (α ? helix content and main bindingpocket) was displayed mainly through spectroscopic experiments: UV-vis, FTIR, Fluorescence, Raman and C.D. It is through these techniques that ahypothesis on the BSA NP assembly was proposed. The toxicity of this NP wastested in an in vivo model: zebrafish (D. rerio). The parameters tested wereteratogenicity, neurotoxicity, cardiotoxicity and phenotypic alterations. Based onthe results sublethal concentrations of the BSA NP were established, and noteratogenicity was observed. On-going implementation research techniqueincludes a biodistribution of the NP in the zebrafish larvae studied by ramanspectroscopy. The BSA NP presented encouraging results to be considered asa nanovehicle for drug delivery systems. It does not only present all thealbumin?s main features as a drug carrier, but also low toxicity levels inzebrafish.This work was supported by grants from Consejo Nacional de InvestigacionesCientíficas y Técnicas, Universidad Nacional de Quilmes, Ministerio de Cienciay Tecnología (MINCyT), Comisión de Investigaciones Científicas de la Provinciade Buenos Aires, Nuclear Atomic Energy Agency (IAEA) CRP codes F220064and F23028.