CONSEQUENCES OF MATERNAL FRUCTOSE INTAKE ON BROWNING POTENTIAL OF RETROPERITONEAL ADIPOSE TISSUE FROM ADULT OFFSPRING

GIOVAMBATTISTA , ANDRÉS; ALZAMENDI, ANA; SPINEDI, EDUARDO

Mar del Plata

Congreso; LXII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2017

SAIC

Beige adipocytes are highly adapted to convert chemical energyinto heat through the action of uncoupling protein-1 (UCP1). Coldexposure or β3 adrenergic agonist treatment stimulates generationof these cells in white adipose tissue (WAT). Our aim was to assesswhether maternal fructose intake during pregnancy, affects browningcapability of retroperitoneal adipose tissue (RPAT) from adultmale offspring. On pregnancy day 1, dams were provided with eithertap water alone (CTR, control) or containing fructose (10%w/v;FRD) and fed ad libitum with chow up to delivery. Lactating damsand their pups (between 21 and 60 days) received water and chowad libitum. C and F indicate pups born to CTR and FRD dams. Onexperimental day (age 60 days) RPAT was dissected and stromalvascular fraction (SVF) cells were isolated. mRNA expression levelsof beige and white adipogenic markers were assessed in RPAT SVFcells and pads. SVF cells were cultured and differentiation parameterswere quantified by qPCR. Previously we found that pre-natalnutritional intervention decreased the adipogenic potential of adultRPAT precursor cells, favoring hypertrophic RPAT expansion anda distorted adipokine secretion pattern. Now, we found that F RPATexpresses lower levels (p