HYBRID PROTEIN-GOLD NANOPARTICLES with bombesin FOR improving cell DELIVERY

GRASSELLI, M.

Maresias

Simposio; 5ª Edição do Workshop de Biomateriais, Engenharia de Tecidos e Orgãos Artificiais; 2017

Sociedad Latinoamericana de Biomateriales y Organos Artificiales

The use of nanotechnology in medicine, also called nanomedicine, is based on the preparaon of nanostructures such as nanoparcles (NPs), with parcular physicochemical characteriscs which can be selected. They can be targeted to specific tumour ssues and modulate their pharmacokinecs, pharmacodynamics and enhanced intracellular acvaon properes. These characteriscs depend on the size and surface properes of the NPs. Albumin-based NPs can be designed for a variety of uses; among them, the ones that stand out are nanovehicles in a drug delivery system material to be used for biocompability of inorganic materials such as gold NPs (Au-NPs). In addion, NP targeng for molecular recognion of anomalous cells could improve the drug delivery efficiency. In this work are shown a preparaon method of core/shell gold/Albumin NP s (Au/Alb-NPs) by desolvaon method and crosslinking by ionizing radiaon. Preparaon of NPs containing an inorganic core such as gold can be very useful for NP tracking and detecon. These Au/Alb-NPs are further decorated with a Bombesin-like pepde for prostate tumoral cell recognion. Extensive characterizaon of the nanoconstructs has been done by LDS, FT-IR spectroscopy, zeta potenal and AFM microscopy analysis. Hybrid NPs in the range of 60-80 nm have been achieved. The nanostructured material was used to study the cellular uptake to PC3 prostate cancer cells. Mullayer Albumin coang shows higher cells uptake than the monolayer protein coated Au-NPs. Further studies should be done to study the cancer cell delivery in viv o . These materials showed promising characteriscs to be used as intracellular delivery carrier of antumoral and/or diagnosis agents.