Studies of Endothelial Monolayer Formation on Irradiated Poly-L-Lactide Acid with Different Ion Energy Densities

CLAUDIA R. ARBEITMAN; MARIELA DEL GROSSO; IRENE IBAÑEZ; MONI BEHAR; MARIANO GRASSELLI; GERARDO GARCÍA BERMÚDEZ

Darmstadt

Conferencia; Swift Heavy Ions in Matter 2015; 2015

GSI Helmholtzzentrum für Schwerionenforschung

In this work we study cell adhesion, proliferation and cell morphology of bovine aortic endothelial cells cultured on poly-L-lactide acid (PLLA) modified by heavy ion irradiation. Previous work [1] compares the results of two beams with about the same stopping power and different ion velocities, therefore with different deposited energy density. They found, that at lower deposited energy density, 20 % increase of cell density and a general better cell morphology. The purpose of the present work is to continue this study and modify the chemically inert PLLA surface with ions with different deposited energy densities. To this end thin films of 50 μm thikness were irradiated with 12C, 16O, 28Si, 32S (2 MeV/amu) and 16O, 63Cu (0.05 up to 0.15 MeV/amu) ion-beams provided by the Tandar (Buenos Aires, Argentina) and Tandetron (Porto Alegre, Brazil) accelerators, respectively. We analyzed the correlation between the stopping power and energy density, with cell metabolism and monolayer formation