Depot-Specific Reprogramming of Adipocyte Precursor Cells (APCs) Induced By Glucocorticoid (GC) Exposure.

ZUBIRIA GUILLERMINA; FRONTINI Y; REY AMANDA; MORENO G; ALZAMENDI, ANA; SPINEDI, EDUARDO; GIOVAMBATTISTA, ANDRÉS

San Diego

Congreso; Endocrine Society's 97th Annual Meeting.; 2015

Endocrine Society

It is well-known that GC excess favors abdominal adipose tissue (AAT), but not subcutaneous adipose tissue (SCAT) accumulation. However, the effect of GC pre-exposition on stromal vascular fraction (SVF) cells from both origins remains unexplored. In the present study we evaluated the impact of dexamethasone (DXM) on the adipogenic potential of APCs from AAT (retroperitoneal) and SCAT (inguinal) pads. For this aim, SVF cells were isolated from AAT and SCAT pads (aseptically dissected from adult male S-D rats), and cultured up to reach confluence. Then DXM (0.25-2.5 µM) was added in culture for 48 h (pre-treatment) and the number of cells with adipogenic potential (defined as CD34+) was determined by flow cytometry. Specific adipocyte competency (PPAR-γ2 and Zfp 423) and, pro- (MR, GR) and anti- (Pref-1, Wnt10b) adipogenic gene markers were quantified in cultured cells (qPCR Real Time). Additionally, induction of differentiation was performed and, parameters of differentiated cells were measured on different differentiation days (Dd): a) adipocyte markers (PPAR-γ2 and C/EBPα, by qPCR) on Dd 4; and b- intracellular lipid content (Oil-Red O) on Dd 10. Our data indicate an increment in PPAR-γ2 gene expression in DXM pre-treated SVFs (Dd 0, P