Trophic factors and chemokine induce chemotactic migration on

JAURENA MB; ZANIN JP; NG CARRI; Y OTROS

Los Cocos, Cordoba

Workshop; WDB; 2006

WDB

Jaurena, MB; Zanín, JP; Carri, NG*; Battiato, NL; Rovasio, RA. Neural crest cells (NCCs) migrate through the embryo body and distribute with precision in “target” fields, where they differentiate in many derivatives. The known factors modulating the dispersion of NCCs are not sufficient to fully explain the oriented migration of this cell population. Here we propose that two trophic factors and a chemokine that are expressed early in development could be involved in directing NCC migration. Using real time video-microscopy and strict directional-based 50 ng/ml SCF (stem cell-derived factor-steel factor) and 300 ng/ml SDF-1 (stromal cell-derived factor1), resembling the results observed with NT-3 (neurotrophin 3). Conclusions: The NCCs respond to SCF and the chemokine SDF-1 with a typical chemotactic behavior, similar to that observed with NT-3. The chemoattractants used here are expresed early in development: SCF in skin and SDF-1 in cefalic region. Likewise, the specific receptor for each factor is expressed in subpopulations of migratory NCCs. SCF could be a posible attractant ciliary ganglion. These data amplify the functional scope of trophic factors and chemokines, integrating them into new directional mechanisms of migratory embryo cells.