NT-3modulate chemotactic migration of neural crest cells through Trks/p75 receptors.

ZANIN P; NG CARRI; R ROVASIO

Los Cocos, Cordoba

Workshop; WDB; 2006

WDB

Introduction: Neural crest cells (NCC) migrate toward specific sites of the embryo and give rise to many derivated.  Although differents molecular factors contribute to cell migration a chemiotactic mechanims could modulate the precise NCC orientation.  In this work, using a chemotactic chamber, videomicroscopy and stric directionally criteria  we show the chemostatic response of mesencephalic NCC migrating up to concentration gradient originated in 40 ng/ml of Neurotrophine 3 (NT3). In addition, we also show a repulsive response from originated in 160 ng/ml of NT-3. Inhibition of Trk’s receptor and specific bloking of P75 antibody, let us to conclude that this receptors are necessary for the directionally response atracction and repulsion of NCC. Conclusions: 1. NT-3 generate in vitro attraction of NCC exposed to low concentration, and repulsion at high concentration of the same factor. 2. This proceses could be initiate in p75 receptors interacting with Trk C for attraction of cells and interacting with other Trk (probably Trk A) to generate the repulsion of the cells.