Study of chromosome damage induced by bleomycin in mammalian cells using FISH technique with telomeric probe?. N.S Paviolo, I.Y Quiroga and A.D Bolzán

N.S PAVIOLO, I.Y QUIROGA AND A.D BOLZÁN

Conferencia; 3rd International Gregorio Weber Conference on New trends in Advanced Fluorescence Microscopy Techniques; 2011

Study of chromosome damage induced by bleomycin in mammalian cells using FISH technique with telomeric probe. N.S Paviolo, I.Y Quiroga, A.D Bolzán. Laboratorio de Citogenética y Mutagenesis, IMBICE, La Plata, Argentina. The effect induced by the radiomimetic compound, bleomycin (BLM) on telomeres was analyzed in the progeny of mammalian cells. The aim of the project was to evaluate if chromosome damage induced at a telomeric level persisted on the progeny of the cell population originally exposed to the mutagen. Fluorescence in situ hybridization (FISH) with the PNA telomeric probe was employed on chromosome preparations of rat cells derived from adipose tissue (ADIPO-P2 cell line). Cells were exposed in the log phase of growth to 2.5 µg/ml of BLM during 30 minutes at 37°C. Cytogenetic analysis revealed a significant increase in the frequency of chromosomal aberrations after 18 h and 10 days after treatment in ADIPO-P2 cells treated with BLM compared with unexposed cells. Our results also indicated that BLM induced delayed telomeric instability in ADIPO-P2 cells, revealed by the presence of incomplete chromosomes and additional telomeric signs in chromosomes of the exposed cells and that the clastogenic effect in vitro of BLM remains at least 10 days after mutagen treatment. Keywords: Telomeres, telomeric instability, mutagen, FISH. Work supported by: CONICET [1] Benkhaled, L., Xunclá, M., Caballín, M.R., Barrios. L., Barquinero, J.F. Induction of complete and incomplete chromosome aberrations by bleomycin in human lymphocytes. Mutat. Res. 637:134-141 (2008). [2] Bolzán, A.D., Bianchi M.S. Detection of incomplete chromosome elements and interstitial fragments induced by bleomycin in hamster cells using a telomeric PNA probe. Mutat. Res. 554:1-8 (2004). [3] Bolzán, A.D., Páez, G.L., Bianchi, M.S. FISH analysis of telomeric repeat sequences and their involvement in chromosomal aberrations induced by radiomimetic compounds in hamster cells. Mutat. Res. 479:187-196 (2001). abolzan@imbice.org.ar. Instituto Multidisciplinario de Biología Celular, (IMBICE), C.C. 403 (1900) La Plata, Argentina.